Showing 34 registered airlines with a combined fleet of 72 airframes.
| Airline Name | ICAO / IATA | Fleet Size |
|---|---|---|
| Civil Aviation Inspectorate of the Czech Republic | CBA | 0 |
| CMA CGM Air Cargo | CMA / 2C | 6 |
| Czech Air Force | CEF | 6 |
| Czech Air Handling | AHD | 0 |
| Czech Airlines | CSA / OK | 3 |
| Czech Government Flying Service | CIE | 0 |
| Eurojet Servis | EJS | 0 |
| Fischer Air | FFR / 8F | 0 |
| Grossmann Jet Service | GSJ | 0 |
| Helicopter | HCP | 0 |